The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
Analysis of Feline Plasma Proteins by Immunoelectrophoresis and Polyacrylamide Gel Electrophoresis
Toshinori FURUKAWAFumihiro SUGIYAMA
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Keywords: feline, IEP, PAGE, plasma protein
JOURNAL FREE ACCESS

1986 Volume 48 Issue 4 Pages 643-653

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Abstract
Twenty-one feline plasma proteins, albumin, α1-acid glycoprotein, α1-antitrypsin, anti-thrombin III, ceruloplasmin, haptoglobin, α2-macroglobulin, hemopexin, transferrin, apolipoprotein (apo) A-I, apo A-II, apo B, apo C-I, apo C-III, immunoglobulin (Ig) G, IgA, IgM, complement (C)3, C4, plasminogen, and fibrinogen, were purified and identified in immunoelectrophoretogram and polyacrylamide gel electrophoretogram. Methods used were polyacrylamide gel electrophoresis (PAGE), SDS-PAGE, agarose electrophoresis, immunoelectrophoresis (IEP), crossed immunoelectrophoresis (CIE), and electro transfer blotting (ETB) for the identification. The positions of 21 feline plasma proteins on immunoelectrophoretogram or polyacrylamide gel electrophoretogram were determined.
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