Ethanol stimulated gluconeogenesis not only from pyruvate, but also from lactate in the isolated liver cells of the eel. Concentrations of lactate and pyruvate were not changed significantly by the addition of ethanol.
Acetate stimulated gluconeogenesis from pyruvate in hepatocytes of the eel. In gluconeogenesis from pyrucate, lactate concentration was increased 2-fold and pyruvate concentration was not changed by the addition of acetate.
Lactate release from the perfused liver in gluconeogenesis from pyruvate was also investigated. Little lactate release was observed.
Effects of ethanol and acetatc on gluconeogenesis in the eel liver was different form that in mammalian liver. The reasons for these differences were discussed in connection with the lactate dehydrogenase in the eel liver.