The wax ester hydrolase activities in liver, hepatopancreas, intestine and pyloric caecum of chub mackerel, blue drum, brown sole, flat fish, yellowtail, red sea bream and black sea bream were compared with the lipase and esterase activities. The lipase activity of pyloric caecum was the highest in all tissues used. In the case of esterase, its activity was remarkable in liver or hepatopancreas of yellowtail and black sea bream, and in pyloric caeca of blue drum and brown sole. Wax ester hydrolysis occurred in almost all the tissues and its hydrolase activity in pyloric caecum of chub mackerel was rather high. The results indicate that wax ester hydrolase activity of the fishes which prey on marine organisms containing more amounts of was esters was noticeable. Pyloric caeca, as a source of lipolytic enzymes, play an important role on wax ester metabolism in the same way as liver, hepatopancreas and intestine.