The fatty acid compositions of the lipids from the fermented sea urchin paste, the salted sea urchin gonads, and the raw sea urchin gonads have been investigated with open-tubular gas-liquid chromatography. All the samples contained about 10.1-17.1% of the unusual 5-olefinic acids such as 5-18:1, 5-20:1, 5, 11-20:2, 5, 13-20:2, 5, 11, 14-20:3, and 5, 11, 14, 17-20:4 and about 0.9-1.3% of the unusual 7-olefinic acids such as 7, 13-22:2 and 7, 15-22:2, similar to the previous studies in our laboratory. The major polyunsaturated acids of these samples were 18:4 n-3 (0.2-6.3%), 20:4 n-6 (4.1-7.6%), and 20:5 n-3 (1.9-17.0%). All the samples showed about 40 species of fatty acid components and various fatty acid compositions. Especially, the salted sea urchin gonads B contained 20:5 n-3, ten times as much as the salted sea urchin gonads A. It was in-ferred that the difference between fatty acid compositions of these samples were related to the fatty acid compositions of the sea urchins which were used as the material.