Two new compounds have been isolated from the pistils and stamens of Hibiscus moscheutos L. and these compounds were determined to be 3, 5, 7, 8, 4'-pentahydroxyflavone 7-O-α-rhamno-pyranoside (VIII) and 2-oxindole-3-acetylaminomethylaspartic acid (X) by chemical and spectroscopic methods. Nine known compounds, β-sitosterol (I), ikshusterol (II), epi-ikshusterol (III), methyl linolenate (IV), β-sitosteyl-β-D-glucoside (V), quercimeritrin (VI), kaempferol-7-O-α-rhamnopyranoside (VII), methyl dioxindole-3-acatate (IX) and rutin (XI), were also isolated.