HeLa cells, spleens and lumbar spinal ganglia of 9-15 day chick embryos are cultured either by Maximow's double cover-slip method or by the roller tube method and the influence of GABA, GABOB and GOBAB upon them are examined. Upon HeLa cells, in the medium (YLE 90%+bovine serum 10%) to which the 3 substances are added each in 5mg/cc, cell proliferation is obviously inhibited in each case. In 0.5mg/cc and 0.05mg/ce, GABOB and GOBAB hardly differ from the control medium while GABA shows a little tendency of promoting prolifera-tion. Upon migrated cells from the spleen, as the result from adding the 3 substances each in 5mg/ce to the medium (Eagle 90%+horse serum 10%), migration of cells is strongly inhibited in each case. In 0.5mg/ce, GABA hardly differs from the control medium, GABOB hastons migration, though slightly, while GOBAB shows the tendency of inhibition. Upon spinal ganglion cells, cultured in the medium (Eagle 90%+horse serum 10%), to which the 3 substances are added in 0.5mg/cc, GABA and GABOB are effective for maintenance and differentiation of nerve cells and for satellite cell association as well, but upon supporting cells, the 3 substances are inhibitory in early stage of culture and GOBAB is rather remarkable of the three.