ウナギ灌流肝における糖新生およびグリコーゲン分解作用

林 征一; 大城 善太郎

doi:10.2331/suisan.41.201

Gluconeogenesis and Glycolysis in Isolated Perfused Liver of the Eel

Seiichi HAYASHI, Zentaro OOSHIRO

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1975 Volume 41 Issue 2 Pages 201-208

DOI https://doi.org/10.2331/suisan.41.201

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Published: February 25, 1975 Received: October 14, 1974 Available on J-STAGE: February 29, 2008 Accepted: - Advance online publication: - Revised: -
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Date of correction: February 29, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : A method for perfusion of isolated eel (Anguilla japonica) liver is described. The perfusion medium employed was Krebs-Ringer bicarbonate without oxygen carriers such as red cells or fluoro carbon and albumin. The rate of gluconeogenesis in excess of lactate was almost the same as that of mammals. Insulin inhibited glycogenolysis of liver glycogen. Epinephrine and 3′, 5′, _-c-AMP stimulated glycogenolysis. Activities of aspartate transami-nase and alanine transaminase were maintained low during perfusion. Oxygen consumption was 115 to 1/7 of that in the perfused rat liver. These results indicate that our perfusion technique provides a useful method for studies of metabolic regulation of eel liver.
Right : A method for perfusion of isolated eel (Anguilla japonica) liver is described. The perfusion medium employed was Krebs-Ringer bicarbonate without oxygen carriers such as red cells or fluoro carbon and albumin. The rate of gluconeogenesis in excess of lactate was almost the same as that of mammals. Insulin inhibited glycogenolysis of liver glycogen. Epinephrine and 3', 5', _-c-AMP stimulated glycogenolysis. Activities of aspartate transami-nase and alanine transaminase were maintained low during perfusion. Oxygen consumption was 115 to 1/7 of that in the perfused rat liver. These results indicate that our perfusion technique provides a useful method for studies of metabolic regulation of eel liver.

Date of correction: February 29, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

A method for perfusion of isolated eel (Anguilla japonica) liver is described. The perfusion medium employed was Krebs-Ringer bicarbonate without oxygen carriers such as red cells or fluoro carbon and albumin. The rate of gluconeogenesis in excess of lactate was almost the same as that of mammals. Insulin inhibited glycogenolysis of liver glycogen. Epinephrine and 3', 5', _-c-AMP stimulated glycogenolysis. Activities of aspartate transami-nase and alanine transaminase were maintained low during perfusion. Oxygen consumption was 115 to 1/7 of that in the perfused rat liver. These results indicate that our perfusion technique provides a useful method for studies of metabolic regulation of eel liver.

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