The sulfate conjugation of phenol and PCP in vitro by liver slices or homogenates from goldfish, carp, rainbow trout, spiny lobster and short-necked clam has been studied and compared with that by albino rat liver, using ³⁵S-labeled K₂SO₄. All the liver slices of the test animals showed sulfate conjugation activity with phenol, the level of activity being 1-30% of that in rat liver (approx. 1.8 μmole/g dry liver/hr). However, the liver homogenates had activities which were markedly lower than those shown by the liver slices.
On the other hand, PCP added to the incubation mixture containing liver slices not only did not conjugate with sulfate, but at concentrations higher than 1.4×10^-6_M, it inhibited the sulfate conjugation of phenol. In short-necked clam and goldfish, absorbed PCP is rapidly excreted after conjugation with sulfate, as reported by K_OBAYASHI et al. (1970 and 1975).