2015 Volume 13 Issue 2 Pages 59-65
In this study, we first investigated the expression of Jagged1 at compression sites during experimental tooth movement in rats in vivo. We then assessed the effects of compression force on Jagged1 production from human periodontal ligament (hPDL) cells.
Twelve 6-week old male Wistar rats were subjected to an orthodontic force of 10 g in order to induce mesial tipping movement of the upper first molars for 7 days. Expression levels of tartrate-resistant acid phosphatase and Jagged1 proteins in the alveolar bone were determined by immunohistochemical analysis. Furthermore, the effects of compression force on Jagged1 production were investigated using hPDL cells in vitro.
Under conditions of experimental tooth movement in vivo, resorption lacunae with multinucleated cells were observed in the 10 g group. In addition, immunoreactivity for Jagged1 was detected on day 7 in PDL tissue subjected to orthodontic force. In the in vitro study, compression force increased the production of Jagged1 from the hPDL cells in a time-dependent manner.
These results suggest that Jagged1 stimulates alveolar bone resorption during orthodontic tooth movement.