Stratum corneum (SC), the outermost skin layer, consists of corneocytes and intercellular lipids. In this study, mechanical properties of a cultured human SC and a tape-stripped human corneocyte were examined in a glycerine solution through atomic force microscopy. A multilayered cultured human SC in the low concentration glycerin solution decreased its Young’s modulus with time. Besides the corneocyte increased its adhesion force in higher concentration of glycerin solution. [DOI: 10.1380/ejssnt.2015.461]