Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan

Juliano de Oliveira PORCIUNCULA; Takanori FURUKAWA; Kazuki MORI; Yosuke SHIDA; Hideki HIRAKAWA; Kosuke TASHIRO; Satoru KUHARA; Satoshi NAKAGAWA; Yasushi MORIKAWA; Wataru OGASAWARA

doi:10.1271/bbb.120794

Biochemistry & Molecular Biology Regular Papers

Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan

Juliano de Oliveira PORCIUNCULA, Takanori FURUKAWA, Kazuki MORI, Yosuke SHIDA, Hideki HIRAKAWA, Kosuke TASHIRO, Satoru KUHARA, Satoshi NAKAGAWA, Yasushi MORIKAWA, Wataru OGASAWARA

Author information

Juliano de Oliveira PORCIUNCULA
Department of Bioengineering, Nagaoka University of Technology
Takanori FURUKAWA
Department of Bioengineering, Nagaoka University of Technology
Kazuki MORI
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Yosuke SHIDA
Department of Bioengineering, Nagaoka University of Technology
Hideki HIRAKAWA
Kazusa DNA Research Institute
Kosuke TASHIRO
Kazusa DNA Research Institute
Satoru KUHARA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Satoshi NAKAGAWA
Kyowa Hakko Bio Co., Ltd.
Yasushi MORIKAWA
Department of Bioengineering, Nagaoka University of Technology
Wataru OGASAWARA
Department of Bioengineering, Nagaoka University of Technology

Keywords: Trichoderma reesei, comparative genomic analysis, cellulase, filamentous fungi, next generation sequencing

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Supplementary material

2013 Volume 77 Issue 3 Pages 534-543

DOI https://doi.org/10.1271/bbb.120794

View "Advance Publication" version (March 7, 2013).

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Abstract

The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of single-nucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.

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