Three strains (K59^T, K60 and K70 ^T) representing two novel yeast species were isolated from the external surface of leaves of different wine grape (Vitis vinifera) plants, which were collected from the Kanchanaburi Research Station (N14°07′15.1″ E099°19′05.6″), Wang Dong Sub-district, Mueang District, Kanchanaburi Province, Thailand, by an enrichment technique. The sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene of two strains (K59^T and K60) were identical and differed from that of strain K70^T. In terms of pairwise sequence similarity of the D1/D2 domain, the closest species to the three strains was Candida asparagi but with 2.3% nucleotide substitutions for strains K59^T and K60, and 2.1% nucleotide substitutions for strain K70^T. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, the three strains were assigned to be two novel Candida species. Two strains (K59^T and K60) were assigned as Candida phyllophila sp. nov. (type strain K59^T=BCC 42662^T=NBRC 107776^T=CBS 12671^T). Candida vitiphila sp. nov. is proposed for strain K70^T (=BCC 42663^T=NBRC 107777^T=CBS 12672^T).