Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
ISSN-L : 1344-7610
Research Papers
Development of EST-SSR markers and construction of a linkage map in faba bean (Vicia faba)
Walid El-Rodeny, Mitsuhiro Kimura, Hideki Hirakawa, Attia Sabah, Kenta Shirasawa, Shusei Sato, Satoshi Tabata, Shigemi Sasamoto, Akiko Watanabe, Kumiko Kawashima, Midori Kato, Tsuyuko Wada, Hisano Tsuruoka, Chika Takahashi, Chiharu Minami, Keiko Nanri, Shinobu Nakayama, Mitsuyo Kohara, Manabu Yamada, Yoshie Kishida, Tsunakazu Fujishiro, Sachiko Isobe
Author information
Keywords: faba bean, EST-SSR, linkage map, comparative mapping, genetic diversity
JOURNAL FREE ACCESS FULL-TEXT HTML
Supplementary material

2014 Volume 64 Issue 3 Pages 252-263

Details
Full-text HTML Download PDF (1542K)
Download citation RIS

(compatible with EndNote, Reference Manager, ProCite, RefWorks)

BIB TEX

(compatible with BibDesk, LaTeX)

Text
How to download citation
Contact us
Abstract

To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a ‘Nubaria 2’ × ‘Misr 3’ F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F2 plants were divided into three subpopulations according to the original F1 plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated ‘Nubaria 2’ × ‘Misr 3’ map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.

Content from these authors
© 2014 by JAPANESE SOCIETY OF BREEDING
Previous article Next article
Favorites & Alerts

Recently viewed articles
Predecessor

Japanese Journal of Breeding

Share this page
feedback
 Top