Rapid and Simple Detection Method of “Candidatus Xenohaliotis californiensis” Using Fecal PCR in Abalone Haliotis discus discus and H. gigantea

Ikunari Kiryu; Toyohiro Nishioka; Kei Yuasa; Jun Kurita; Yoshik Shimahara; Mitsuru Ototake; Naoya Ikegami; Norihisa Oseko

doi:10.3147/jsfp.49.41

Research Article

Rapid and Simple Detection Method of “Candidatus Xenohaliotis californiensis” Using Fecal PCR in Abalone Haliotis discus discus and H. gigantea

Ikunari Kiryu, Toyohiro Nishioka, Kei Yuasa, Jun Kurita, Yoshik Shimahara, Mitsuru Ototake, Naoya Ikegami, Norihisa Oseko

Author information

Keywords: withering syndrome, Candidatus Xenohaliotis californiensis, abalone, feces, boiling extraction, fecal PCR, screening

JOURNAL FREE ACCESS

2014 Volume 49 Issue 2 Pages 41-48

DOI https://doi.org/10.3147/jsfp.49.41

Details

Abstract

We developed a simple PCR-based method using feces to detect “Candidatus Xenohaliotis californiensis” (Rickettsia-like organism, RLO), which is responsible for withering syndrome (WS). Four abalone groups (Haliotis discus discus and H. gigantea) naturally or artificially infected with WS-RLO were prepared. After daily collection of the feces from individual animals over a seven-day period, the posterior esophagus (PE) was excised, and subjected to PCR for WS-RLO. WS-RLO-positive results were obtained from the feces of 30-67% animals, and from the PE of 17-97% animals. For the fecal PCR, more than one animal was positive from each group every day, although the WS-RLO-positive rate daily varied. When the sensitivity of the PCR was compared between DNA extracted from feces by boiling and that by QIAamp^® DNA Stool Kit, there was no difference between the two methods. Fecal PCR combined with boiling DNA extraction is rapid and simple for screening abalone groups infected with WS-RLO.

Corresponding author

Register with J-STAGE for free!