Laser manipulation-microfabrication system consisting of a CW laser, a picosecond pulsed laser and an optical microscope was developed to assemble micrometer-sized structure in solution. Photopolymerization of reactive monomers induced at the focal spot of picosecond laser pulses (λ = 355 nm or 532 nm, FWHM = 30 ps, repetation rate = 10 Hz) was employed for connection of glass particles regularly aligned by laser trapping. Since photopolymerization was induced in the vicinity of a focal spot, any desired numbers of glass particles with arbitrary geometrical structure can be connected by repeating the manipulation and the photopolymerization procedures.