Polysaccharides widely used in the food industry were studied in terms of sterilization of bacteria by irradiation.12 items of polysaccharides irradiated by electron beam ware investigated for bacteria count and the functional property of pH, gel strength, bloom and viscosity. This study aims to determine the sterilization effectby absorption dose and the applicability of the electron beam irradiation toward polysaccharides. Results shows that 1) Over 5kGy absorption dose are enough to be able to sterilize bacteria in the polysaccharidethemselves. 2) We reconfirm that Arabic gum will be applicable for the electron beam irradiation, which hasbeen used in some foreign countries. 3) Electron beam irradiation will be useful for Gellan gum b (acetyl type), as gelling agents in the food application.