The advanced laser techniques for multiphoton microscope are introduced in terms of femtosecond pulse generation. The compact ultrafast fiber lasers are described as the second generation of femtosecond lasers for microscopy. For the third generation techniques, the advanced techniques such as light propagation control by index profiles in core and clad area are promising. The photonic fiber structure generates the super continuum in 1-m long fiber. Several ideas for multi-color and tunable pulse generation are discussed. The engineering developed for scientific and industrial applications should be combined for the compact and reliable light source for multiphoton microscopy.