Pili of Vibrio cholerae Widely Distributed in Serogroup O1 Strains
1993 Volume 37 Issue 1 Pages 23-28
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1993 Volume 37 Issue 1 Pages 23-28
The distribution of Vibrio cholerae O1 pili consisting of 16kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.
