1993 Volume 40 Issue 3 Pages 311-314
Laminaribiose was synthesized from sucrose by coupling reactions of sucrose phosphorylase, xylose isomerase and laminaribiose phosphorylase in the presence of a catalytic amount of Pi. For the synthesis, a reaction mixture (2ml) containing 200 mM sucrose, 20 mM Pi, 0.47 U/ml sucrose phosphorylase, 0.072 U/ml xylose isomerase, and 0.37 U/ml Laminaribiose phosphorylase in 50 mM imidazole-HCl buffer (pH 7.0) was used. The concentration of Laminaribiose reached 110 mM after 48 hr incubation at 37°C. Laminaritriose was also formed in the same mixture after 15 hr, and its concentration reached 22 mM.