Journal of the Japanese Society of Starch Science
Online ISSN : 1884-488X
Print ISSN : 0021-5406
ISSN-L : 0021-5406
Conversion of Sucrose into Laminaribiose Using Sucrose Phosphorylase, Xylose Isomerase and Laminaribiose Phosphorylase
Motomitsu KITAOKATakashi SASAKIHajime TANIGUCHI
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JOURNAL FREE ACCESS

1993 Volume 40 Issue 3 Pages 311-314

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Abstract

Laminaribiose was synthesized from sucrose by coupling reactions of sucrose phosphorylase, xylose isomerase and laminaribiose phosphorylase in the presence of a catalytic amount of Pi. For the synthesis, a reaction mixture (2ml) containing 200 mM sucrose, 20 mM Pi, 0.47 U/ml sucrose phosphorylase, 0.072 U/ml xylose isomerase, and 0.37 U/ml Laminaribiose phosphorylase in 50 mM imidazole-HCl buffer (pH 7.0) was used. The concentration of Laminaribiose reached 110 mM after 48 hr incubation at 37°C. Laminaritriose was also formed in the same mixture after 15 hr, and its concentration reached 22 mM.

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© The Japanese Society of Applied Glycoscience
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