Rapid and Sensitive PCR Detection of Vibrio trachuri Pathogenic to Japanese Horse Mackerel (Trachurus japonicus)

Yoshihisa Iwamoto; Yusaku Suzuki; Akinobu Kurita; Yoko Watanabe; Tadayori Shimizu; Hirohisa Ohgami; Yasutake Yanagihara

Rapid and Sensitive PCR Detection of Vibrio trachuri Pathogenic to Japanese Horse Mackerel (Trachurus japonicus)

Yoshihisa Iwamoto, Yusaku Suzuki, Akinobu Kurita, Yoko Watanabe, Tadayori Shimizu, Hirohisa Ohgami, Yasutake Yanagihara

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Keywords: Polymerase chain reaction, Vibrio trachuri, Japanese horse mackerel, Pst I fragment

JOURNAL FREE ACCESS

1995 Volume 39 Issue 12 Pages 1003-1006

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Abstract

A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specifically amplified the DNAs from V. trachuri T9210, T9213, and T9216 but not of those other bacterial strains. PCR using a Pst I-1 primer set made it possible to detect 100fg of T9210 DNA. The PCR method reported here may be useful for detection and identification of V. trachuri pathogenic to Japanese horse mackerel.

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