Differential Expression of a Mycobacterium tuberculosis Protein Recognized by a Mouse Monoclonal Antibody
1996 Volume 40 Issue 11 Pages 883-886
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1996 Volume 40 Issue 11 Pages 883-886
Murine monoclonal antibodies were produced against Mycobacterium tuberculosis (Mtb) using standard hybridoma procedures. By a whole cell enzyme-linked immunosorbent assay (ELISA), one monoclonal antibody (mAb), HB28, demonstrated high level specific reactivity to Mtb. Western blot analysis demonstrated reactivity to a single 65kDa Mtb protein in the cell wall extract and culture filtrate. HB28 mAb appears to be recognizing a 65kDa Mtb protein that is over-expressed by Mtb but not other species under certain culture conditions. Differential expression and detection of this protein by HB28 mAb may have potential for diagnostic applications.
