A Structural Analysis of the Regularly Arranged Porin on the Outer Membrane of Campylobacter jejuni Based on Correlation Averaging

Kazunobu Amako; Norio Baba; Nobuyuki Suzuki; Sun Nyunt Wai; Akiko Umeda

A Structural Analysis of the Regularly Arranged Porin on the Outer Membrane of Campylobacter jejuni Based on Correlation Averaging

Kazunobu Amako, Norio Baba, Nobuyuki Suzuki, Sun Nyunt Wai, Akiko Umeda

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Keywords: Porin, Campylobacter jejuni, Outer membrane, Correlation averaging, Computer, Electron microscopy, Negative staining

JOURNAL FREE ACCESS

1997 Volume 41 Issue 11 Pages 855-859

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Abstract

A negatively stained electron micrograph of regularly arranged porin proteins of Campylobacter jejuni on the isolated outer membrane of bacteria was analyzed in detail by the correlation averaging method using a computer-assisted program. The results showed that the porin of C. jejuni had a trimeric structure separated by about 10.4±0.15nm. In addition, the pores in the trimers were also separated by about 4.3±0.1nm.

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