Using microscale multisample two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) in the absence of denaturing agents, eight protein samples can be simultaneously analyzed and compared. In the present study, this method was applied for the analysis of chicken serum, chicken egg yolk and chicken egg albumen proteins, and the 2-D PAGE pattern of chicken yolk and albumen were compared with that of chicken serum. The spot locations (pI and Mr) of yolk proteins resembled with that of chicken serum, but those of albumen proteins were considerably different from those of chicken serum. Employing the techniques of electrophoretic blotting and immunochemical staining, immunoglobulin G (IgG), third component of complement (C3) and transferrin were identified in the patterns of chicken serum and yolk proteins.