Apolipoprotein C-II (apo C II), a protein constituent of human very low density lipoproteins, is the activator for lipoprotein lipase (LPL). It had been established that apo C II has amino acid sequence of the 78 residues.
To determine the minimal sequence requirements for activation, we have prepared synthetic fragments of apo C II and tested them for their ability to activate LPL. The COOH-terminal synthetic fragment 76-78, 75-78, 74-78, 73-78, 72-78 were tested.
All fragements had a little ability to activate LPL. How ever, the fragment corresponding to residue 75-78 increased the hydrolysis more significantly to compare with other synthetic fragments.
