Monoclonal Antibodies to O4 Antigen of Vibrio parahaemolyticus

Setsuko OGAWA; Tomihiko KOSHIKAWA; Kazuhito WATABE; Mari YAMAZOE; Mitsuo TORII; Nobuo SAKATO; Takachika AZUMA; Hajime FUJIO

Monoclonal Antibodies to O4 Antigen of Vibrio parahaemolyticus

Setsuko OGAWA, Tomihiko KOSHIKAWA, Kazuhito WATABE, Mari YAMAZOE, Mitsuo TORII, Nobuo SAKATO, Takachika AZUMA, Hajime FUJIO

Author information

Setsuko OGAWA
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University
Tomihiko KOSHIKAWA
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University
Kazuhito WATABE
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University
Mari YAMAZOE
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University
Mitsuo TORII
Department of Environmental Health Sciences, Faculty of Pharmaceutical Sciences, Setsunan University
Nobuo SAKATO
Department of Immunology, The Research Institute for Microbial Diseases, Osaka University
Takachika AZUMA
Department of Immunology, The Research Institute for Microbial Diseases, Osaka University
Hajime FUJIO
Department of Immunology, The Research Institute for Microbial Diseases, Osaka University

JOURNAL FREE ACCESS

1988 Volume 32 Issue 2 Pages 211-220

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Abstract

Four monoclonal antibodies were prepared against O4 antigen of Vibrio parahaemolyticus. All the antibodies were shown to be specific for O4 antigen by agglutination with heat-killed O-cells of the organism and precipitation with LPS preparations. The inhibition experiments of the precipitations with various sugars and oligosaccharides suggested that the combining sites of these hybridoma antibodies were directed to an antigenic determinant structure containing →3 and →6 linked D-glucose, D-galactose, and N-acetyl-D-galactosamine.

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