Expression of Notch Signal in the Regenerated Olfactory Epithelium after Damage

Yorihisa Orita; Hitoshi Nagatsuka; Junko Yoshinobu; Hidetsugu Tsujigiwa; Yukihide Maeda; Masashi Kakiuchi; Saeko Orita; Ayako Takeuchi; Yasushi Takeda; Noriyuki Nagai; Kazunori Nishizaki

doi:10.7248/jjrhi1982.44.2_114

Yorihisa Orita, Hitoshi Nagatsuka, Junko Yoshinobu, Hidetsugu Tsujigiwa, Yukihide Maeda, Masashi Kakiuchi, Saeko Orita, Ayako Takeuchi, Yasushi Takeda, Noriyuki Nagai, Kazunori Nishizaki

Author information

Yorihisa Orita
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Hitoshi Nagatsuka
Oral Pathology Medicine, Okayama School of Medicine and Dentistry
Junko Yoshinobu
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Hidetsugu Tsujigiwa
Oral Pathology Medicine, Okayama School of Medicine and Dentistry
Yukihide Maeda
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Masashi Kakiuchi
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Saeko Orita
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Ayako Takeuchi
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Yasushi Takeda
Department of Otolaryngology-Head and Neck Surgery, Okayama School of Medicine and Dentistry
Noriyuki Nagai
Oral Pathology Medicine, Okayama School of Medicine and Dentistry
Kazunori Nishizaki
Oral Pathology Medicine, Okayama School of Medicine and Dentistry

Keywords: Notch Signal, in situ hybridization

JOURNAL FREE ACCESS

2005 Volume 44 Issue 2 Pages 114-118

DOI https://doi.org/10.7248/jjrhi1982.44.2_114

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Abstract

We studied the expression of Notch1, Math1, Jagged2 and Hes5 in the regenerated olfactory epithelium (OE) of adult mice. The OE was first destroyed by intranasal zinc sulfate irrigation and, after 7days, some regenerated areas were observed by in situ hybridization (ISH). In mice treated with zinc sulfate, Notch1 expression was sparsely observed in the regenerated OE and the number of Notch1-positive cells appeared to be lower than that in the OE of control cases. Math1 and Jagged2 expression was observed in the regenerated OE. These expression patterns appear to be the same as those in control cases. No Hes5 was observed in either the normal or regenerated OE. We concluded that Notch1 down-regulation in the damaged OE may play an important role in OE regeneration.

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