The estimation of changes in human uptake of dioxins in Tokyo Bay basin as a consequence of the reduction of Co-PCB emissions into the environment following the implementation of the Law Concerning Special Measures for Promotion of Proper Treatment of PCB Waste was discussed. Firstly, chronological decreases in Co-PCB concentrations in sediment were estimated, considering the reduction of Co-PCB emission using a multimedia environmental model. Secondly, Co-PCB concentrations in fish in Tokyo Bay were estimated on the basis of the concentrations in sediment and Biota Sediment Accumulation Factors (BSAF). Lastly, the total daily intake (TDI) of Co-PCB, Dibenzo-p-dioxins and Dibenzofurans was chronologically projected considering the daily intakes of these chemicals from fish based on previously estimated concentrations in fish in Tokyo Bay and in other areas. As a result, the TDI in Tokyo Bay Basin in 2016, the target year of the PCB Law, was estimated to be about 1pg-TEQ/(kg-weight · day) .