Early Detection of the Parasitic Copepode Pectenophilus ornatus in the Gills of the Japanese Scallop (Patinopecten yessoensis) by Using Polymerase Chain Reaction with Species-specific Primers

Hidekatsu SUZUKI; Takeshige MATSUTANI; Hidetoshi TAMATE

doi:10.11233/aquaculturesci1953.54.353

Early Detection of the Parasitic Copepode Pectenophilus ornatus in the Gills of the Japanese Scallop (Patinopecten yessoensis) by Using Polymerase Chain Reaction with Species-specific Primers

Hidekatsu SUZUKI, Takeshige MATSUTANI, Hidetoshi TAMATE

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Keywords: Pectenophilus ornatus, Patinopecten yessoensis, Specific-primer, PCR products

JOURNAL FREE ACCESS

2006 Volume 54 Issue 3 Pages 353-357

DOI https://doi.org/10.11233/aquaculturesci1953.54.353

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Abstract

Partial sequences of 18S ribosomal RNA genes were amplified by using the polymerase chain reaction (PCR) with template DNA extracted from a parasitic copepode, Pectenophilus ornatus, and its host Japanese scallop, Patinopecten yessoensis. Species-specific internal primers were designed based upon the characteristic nucleotide sequence of P. ornatus, which allowed detection of this species in the early stage of infection by the amplicon size of the PCR product.

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