Enhancement of Anti-tumor Cytotoxicity of Expanded γδ T Cells by Stimulation with Monocyte-derived Dendritic Cells

Anri Saito; Miwako Narita; Ayumi Yokoyama; Norihiro Watanabe; Nozomi Tochiki; Noriyuki Satoh; Jun Takizawa; Tatsuo Furukawa; Ken Toba; Ichiro Fuse; Yoshifusa Aizawa; Shohji Shinada; Masuhiro Takahashi

doi:10.3960/jslrt.47.61

Anri Saito, Miwako Narita, Ayumi Yokoyama, Norihiro Watanabe, Nozomi Tochiki, Noriyuki Satoh, Jun Takizawa, Tatsuo Furukawa, Ken Toba, Ichiro Fuse, Yoshifusa Aizawa, Shohji Shinada, Masuhiro Takahashi

Author information

Anri Saito
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Division of Hematology, Graduate School of Medical and Dental Sciences, Niigata University
Miwako Narita
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Ayumi Yokoyama
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Norihiro Watanabe
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Division of Hematology, Graduate School of Medical and Dental Sciences, Niigata University
Nozomi Tochiki
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Noriyuki Satoh
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University
Jun Takizawa
Division of Hematology, Graduate School of Medical and Dental Sciences, Niigata University
Tatsuo Furukawa
Division of Stem Cell Transplantation, Niigata University Medical and Dental General Hospital, Niigata University
Ken Toba
Division of Hematology, Graduate School of Medical and Dental Sciences, Niigata University
Ichiro Fuse
Bioscience Medical Research Center, Niigata University Medical and Dental General Hospital, Niigata University
Yoshifusa Aizawa
Division of Hematology, Graduate School of Medical and Dental Sciences, Niigata University
Shohji Shinada
Niigata Prefecture Red Cross Blood Center
Masuhiro Takahashi
Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University

Keywords: γδ T cells, monocyte-derived dendritic cells, CD69, anti-tumor cytotoxicity, cellular immunotherapy

JOURNAL FREE ACCESS

2007 Volume 47 Issue 2 Pages 61-72

DOI https://doi.org/10.3960/jslrt.47.61

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Abstract

In order to establish the method of generating powerful γδ T cells for anti-tumor immunotherapy, we investigated the effects of monocyte-derived dendritic cells (mo-DCs) on anti-tumor cytotoxicity of expanded γδ T cells. Activation of γδ T cells co-cultured for 2-3 days with immature or mature mo-DCs was evaluated by CD69 expression and anti-tumor cytotoxicity using two assays : the 5- (and 6-) carboxyfluorescein diacetate, succinimidyl ester-based cytotoxicity assay and the calcein-AM-based Terascan assay. γδ T cells were used as effector cells and myeloma cell line (RPMI8226) or chronic myelogenous leukemia blastic crisis cell line (C2F8) were used as target cells. CD69 expression on γδ T cells was enhanced by co-culture with both immature and mature mo-DCs in a cell-number-dependent fashion. CD69 expression was enhanced after addition of mo-DCs of either autologous or allogeneic origin. Activation of γδ T cells with mo-DCs enhanced anti-tumor cytotoxicity of γδ T cells against RPMI8226 and C2F8 in an effector-to-target ratio-dependent manner. Activation of γδ T cells by mo-DCs was associated with the enhancement of anti-tumor cytotoxicity of γδ T cells. Potent γδ T cells activated by mo-DCs were considered to be applicable to an efficient γδ T cell-mediated immunotherapy for tumors. [J Clin Exp Hematopathol 47(2) : 61-72, 2007]

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