Official Journal of Japan Society of Ningen Dock
Online ISSN : 2186-5027
Print ISSN : 1880-1021
ISSN-L : 1880-1021
Evaluation of Immunoglobulin G Antibody Titer Measurement in the Simplified Test for Multiple Bacterial Infection in Periodontal Disease Based on Self-Sampling of Fingertip Capillary Blood
-Focusing on Porphyromonas gingivalis Antigen-
Eisuke Maehata, Yojiro Maehata, Masaichi-Cong-il Lee, Chieko Kudo, Shogo Takashiba, Hiroji Shimomura, Minoru Yamakado, Masao Yano, Teruo Shiba, Ikuo Hatakeyama, Minoru Inoue, Kunio Kouka, Tetsuo Adachi, Naoya Kishikawa, Naotaka Kuroda, Shinya Sugimoto, Hiromi Watanabe, Kazumasa Koga, Naoko Ikoshi, Katsuhisa Shimizu
Author information
Keywords: bacteriological test for periodontal disease, Porphyromonas gingivalis (Pg), immunoglobulin G (IgG) antibody titer, fingertip capillary blood
JOURNAL FREE ACCESS

2008 Volume 22 Issue 6 Pages 35-41

Details
Download PDF (1806K)
Download citation RIS

(compatible with EndNote, Reference Manager, ProCite, RefWorks)

BIB TEX

(compatible with BibDesk, LaTeX)

Text
How to download citation
Contact us
Abstract

Background Periodontal disease is a multiple infection caused by bacteria represented by Porphyromonas gingivalis (Pg). The prevalence of periodontal disease is high, as it predominantly affects the people in the same age range as diabetes mellitus, but it is often overlooked because of the lack of subjective symptoms. There has been a need for the introduction of self-sampled device-treated plasma using fingertip capillary blood in routine tests.
Methods Based on the report by Kudo et al. (Beppu Conference 2006, p.46, 2006) on enzyme-linked immunosorbent assay (ELISA) as a blood test for periodontal disease bacteria, the precision and disease specificity were examined towards the establishment of a methodology for routine tests.
Results The intra-assay reproducibility of the measurements using Pg and 3 other types of antigens, Prevotella intermedia (Pi), Eikenella corroders (Ec), and Actinobacillus actinomycetemcomitans (Aa), was coefficient of variation (CV) 4-7%, and the results from capillary blood and those from venous blood were closely analogous to each other with a correlation (r) of 0.900 or more. The difference between the non-periodontal control subjects group and the periodontal disease group in the distribution of data on histograms using the Pg antigen was approximately 7-fold. In addition, the correlation with mean periodontal pocket depth was significant (r=0.597, p<0.001) in the group showing the test results (SD index; SDI) of 20 or more. These results substantiated the specificity of this method.
Conclusion The ability to detect periodontal disease in the setting of “Human Dry Dock” screening examinations using the sampling and test methods proposed by us would be an important means to improve services. This study established the practical feasibility of this approach.

Content from these authors
© Japan Society of Ningen Dock
Previous article Next article
Favorites & Alerts

Recently viewed articles
Predecessor

Official Journal of the Japanese Society of Human Dry Dock

Share this page
feedback
 Top