1. Histidine peptides derived from horse globin with Streptomyces griseus proteinase, were separated on a column of Amberlite IRC-50 (pH 5.2) and purified as the α-mono-TNP-derivatives.
2. On the acid-hydrolysis of α-mono-TNP-histidine peptides, α-mono-TNP-histidine from N-terminal and free histidine from the non-N-terminal positions, could be recovered in good yield, respectively.
3. The presences of 4 moles of His-Ala-Ser, 3 moles of His-Ser-Gly, one mole of His-His-Gly, Ala-His-His-Arg, and Ser-His per 35, 000g. of horse globin was elucidated.
The present work was aided in part by the Scientific Research Grant from the Mini-stry of Education.