The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

Md. Anamul HOQUE; Misugi URAJI; Mst. Nasrin Akhter BANU; Izumi C. MORI; Yoshimasa NAKAMURA; Yoshiyuki MURATA

doi:10.1271/bbb.100393

Biochemistry & Molecular Biology Notes

The Effects of Methylglyoxal on Glutathione S-Transferase from Nicotiana tabacum

Md. Anamul HOQUE, Misugi URAJI, Mst. Nasrin Akhter BANU, Izumi C. MORI, Yoshimasa NAKAMURA, Yoshiyuki MURATA

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Keywords: methylglyoxal, glutathione S-transferase, glutathione, thiol, 1-chloro-2,4-dinitrobenzene

JOURNAL FREE ACCESS

2010 Volume 74 Issue 10 Pages 2124-2126

DOI https://doi.org/10.1271/bbb.100393

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Abstract

Methylglyoxal (MG) is one of the aldehydes that accumulate in plants under environmental stress. Glutathione S-transferases (GSTs) play important roles, including detoxification, in the stress tolerance systems of plants. To determine the effects of MG, we characterized recombinant GST. MG decreased GST activity and thiol contents with increasing K_m. GST can serve as a target of MG modification, which is suppressed by application of reduced glutathione.

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