An Enzymatic Colorimetric Quantification of Orthophosphate

Bingxue Li; Takanori Nihira; Hiroyuki Nakai; Mamoru Nishimoto; Motomitsu Kitaoka

doi:10.5458/jag.jag.JAG-2011_002

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An Enzymatic Colorimetric Quantification of Orthophosphate

Bingxue Li, Takanori Nihira, Hiroyuki Nakai, Mamoru Nishimoto, Motomitsu Kitaoka

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Keywords: colorimetric quantification, enzymatic quantification, orthophosphate, peroxidase, pyruvate oxidase

JOURNAL FREE ACCESS

2011 Volume 58 Issue 3 Pages 125-127

DOI https://doi.org/10.5458/jag.jag.JAG-2011_002

View "Advance Publication" version (June 9, 2011).

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Abstract

Quantification of orthophosphate (Pi) in the presence of labile phosphate esters is required for biochemical assays. We developed a method for the enzymatic colorimetric quantification of Pi using pyruvate oxidase and peroxidase. The calibration curve was not affected by the presence of labile phosphate esters. Furthermore, this method allows continuous monitoring of the reaction of Pi-releasing enzymes.

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