We have recently realized the two-dimensional visualization of a oligoethyleneglycol (OEG)/mannose (Man)-terminated pattern surface by performing AFM force measurements using a concanavalin A (ConA)-modified AFM tip. To extend the visualization technique, various types of patterned surfaces with localized sugar chains were fabricated by lithography and their two-dimensional visualizations were challenged in a phosphate-buffered saline solution by AFM force measurements using a ConA-modified AFM tip in this study. The adhesion force based on the interaction of ConA with Man was much larger than that based on the interaction of ConA with phosphorylcholine or galactose. The spatial resolution of this method was evaluated using a nanopatterned surface fabricated using silica nanoparticles. Man-terminated regions were clearly distinguishable from OEG-terminated regions on a nanometer scale.