Transcriptomic evaluation of the enhanced plant growth-inhibitory activity caused by derivatization of cis-cinnamic acid

Naoya Wasano; Mami Sugano; Keisuke Nishikawa; Katsuhiro Okuda; Mitsuru Shindo; So-Young Park; Syuntaro Hiradate; Tsunashi Kamo; Yoshiharu Fujii

doi:10.1584/jpestics.D13-090

Original Articles

Transcriptomic evaluation of the enhanced plant growth-inhibitory activity caused by derivatization of cis-cinnamic acid

Naoya Wasano, Mami Sugano, Keisuke Nishikawa, Katsuhiro Okuda, Mitsuru Shindo, So-Young Park, Syuntaro Hiradate, Tsunashi Kamo, Yoshiharu Fujii

Author information

Naoya Wasano
Biodiversity Division, National Institute for Agro-Environmental Sciences
Mami Sugano
Biodiversity Division, National Institute for Agro-Environmental Sciences
Keisuke Nishikawa
Institute for Materials Chemistry and Engineering, Kyushu University
Katsuhiro Okuda
Institute for Materials Chemistry and Engineering, Kyushu University
Mitsuru Shindo
Institute for Materials Chemistry and Engineering, Kyushu University
So-Young Park
Biodiversity Division, National Institute for Agro-Environmental Sciences
Syuntaro Hiradate
Biodiversity Division, National Institute for Agro-Environmental Sciences
Tsunashi Kamo
Biodiversity Division, National Institute for Agro-Environmental Sciences
Yoshiharu Fujii
Biodiversity Division, National Institute for Agro-Environmental Sciences

Keywords: cis-cinnamic acid analogues, Arabidopsis thaliana, DNA microarray, Gene Ontology analysis, structure-bioactivity relationship, auxin

JOURNAL FREE ACCESS FULL-TEXT HTML
Supplementary material

2014 Volume 39 Issue 2 Pages 85-90

DOI https://doi.org/10.1584/jpestics.D13-090

Browse “Advance online publication” version

Details

Abstract

To establish a rapid high-throughput evaluation system for the enhanced plant growth-inhibitory activity caused by modifications of cis-cinnamic acid’s (cis-CA’s) chemical structure, a DNA microarray assay was used to analyze the changes in early gene responses of Arabidopsis thaliana seedlings. After a 6-hr exposure to (Z)-3-(3-iodophenyl)acrylic acid, we observed an upregulation in three classes of early auxin-responsive genes, which was similar to the transcriptional response to indole-3-acetic acid (IAA), together with an upregulation of the genes related to environmental stress and toxin detoxification responses. Gene responses to 2-(3,4-dihydronaphthalen-1-yl)acetic acid were similar to those to IAA. In contrast, fewer genes were upregulated in response to its double-bond isomer, (Z)-2-[3,4-dihydronaphthalen-1(2H)-ylidene]acetic acid, than to cis-CA. DNA microarray data suggest that the structurally different cis-CA analogues trigger diverse gene responses.

Corresponding author

Register with J-STAGE for free!