In Lake Akan, Hokkaido, northern Japan, the local population of the native and endangered Japanese crayfish Cambaroides japonicus (De Haan, 1841) is extinct, whereas the signal crayfish Pacifastacus leniusculus (Dana, 1852), an invasive species from North America, has become established and rapidly increased. This study provides information about specimens of C. japonicus preserved by Dr. Saburo Hatta from the lake in 1872. The six specimens (two males and four non-ovigerous females) are valuable evidence of C. japonicus formerly inhabiting Lake Akan. The body sizes of the specimens were 24.4–29.5 mm in carapace length and 55.7–67.4 mm in total length, and the estimated age of the largest specimen was ten years, indicating that Lake Akan of that time provided a suitable habitat. Twenty-two ectosymbiotic crayfish worms (Annelida, Clitellata, Branchiobdellida) were found attached to the specimens. Three branchiobdellidan species were identified: Cirrodrilus cirratus Pierantoni, 1905, C. inukaii (Yamaguchi, 1934) and C. megalodentatus (Yamaguchi, 1934). This is the first record of the latter two species from Lake Akan.