Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase

Koichi Abe; Masahiro Nakajima; Motomitsu Kitaoka; Hiroyuki Toyoizumi; Yuta Takahashi; Naohisa Sugimoto; Hiroyuki Nakai; Hayao Taguchi

doi:10.5458/jag.jag.JAG-2014_011

Regular Papers

Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase

Koichi Abe, Masahiro Nakajima, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, Hayao Taguchi

Author information

Koichi Abe
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
Masahiro Nakajima
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
Motomitsu Kitaoka
National Food Research Institute, National Agriculture and Food Research Organization
Hiroyuki Toyoizumi
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
Yuta Takahashi
Graduate School of Science & Technology, Niigata University
Naohisa Sugimoto
Graduate School of Science & Technology, Niigata University
Hiroyuki Nakai
Graduate School of Science & Technology, Niigata University
Hayao Taguchi
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science

Keywords: 1,2-β-glucan, 1,2-β-oligoglucan phosphorylase, sucrose phosphorylase, glycoside hydrolase family 94

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Supplementary material

2015 Volume 62 Issue 2 Pages 47-52

DOI https://doi.org/10.5458/jag.jag.JAG-2014_011

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Abstract

1,2-β-Glucan was produced enzymatically from 1.0 M sucrose and 0.5 M glucose by the combination of sucrose phosphorylase and 1,2-β-oligoglucan phosphorylase in the presence of 100 mM inorganic phosphate. Accumulation of 1,2-β-glucan in 2 L of the reaction mixture reached over 800 mM (glucose equivalent). Sucrose, glucose and fructose were removed after the reaction by yeast treatment. 1,2-β-Glucan was precipitated with ethanol to obtain 167 g of 1,2-β-glucan from 1 L of the reaction mixture.

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