We propose a novel fabrication methodology for a hermetic sealing device using an O₂ plasma-assisted low temperature Intrinsic-Silicon (I-Si) and glass bonding technique. Glass substrates were used as the cap and base wafers, while I-Si was applied selectively to the contact surface of the cap wafer as an interlayer. A 180-μm-deep cavity was formed in the cap glass by wet etching using a double-layer (photoresist/I-Si) etching mask. I-Si/glass bonding was conducted at 200°C using an I-Si layer-covered glass wafer and a bare glass wafer. Water contact angle measurements and tensile test results showed that the bonding strength increased with promoting surface hydrophilicity through O₂ plasma pretreatment. Moreover, scanning acoustic microscope observations revealed that I-Si/glass bonding was achieved successfully without significant voids. Our results indicate that the proposed method could become an indispensable technique for future functional hermetic sealing devices.